mechanism-erectile-dysfunction-research

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Study of functioning of same key protein that is seen in erectile dysfunction as well as cardiovascular system functions

The following is study done by researchers in Germany’s Hannover Medical School’s Division of Surgery, Department of Urology and Urological Oncology.

Can we use medicines such as Viagra © for prevention and or cure of heart attack, stroke?

We study to search if the same molecules are at play in heart muscle infarction and erectile dysfunction.

This is one such study that shows that a (cAK) key protein that affects functions of the cardiovascular system also affects blood supply in penis resulting in Erectile Dysfunction.

We are fascinated by medicines that improve erectile dysfunction because we wonder if the same medicines can be useful in improving blood supply to heart, brain and other organs just as they do for penis.

The artilce begins here:
Source:
Journal of Sexual Medicine

Expression of Cyclic AMP-dependent Protein Kinase Isoforms in Human Cavernous Arteries: Functional Significance and Relation to Phosphodiesterase Type 4

Eginhard S. Waldkirch, MD,* Stefan Ückert, PhD,* Katja Sigl, PhD, † Imke Satzger, MD, ‡ Ulrike Geismar, MD, § Kristina Langnäse, PhD, ¶ Karin Richter, PhD, ¶ Michael Sohn, MD,** Markus A. Kuczyk, MD, PhD,* and Petter Hedlund, MD, PhD ††
*Hannover Medical School—Division of Surgery, Department of Urology and Urological Oncology, Hannover, Germany; † MorphoSys AG, Martinsried, Germany; ‡ Hannover Medical School—Department of Dermatology and Allergology, Hannover, Germany; § Private Dermatological Practice, Hannover, Germany; ¶ Otto-von-Guericke-University, Faculty of Medicine—Institute for Biochemistry and Cell Biology, Magdeburg, Germany; **Frankfurter Diakonie-Kliniken, St. Markus Academic Hospital—Department of Urology, Frankfurt am Main, Germany; †† University Vita Salute, Faculty of Medicine, San Raffaele Hospital—Department of Urology, Urological Research Institute, Milan, Italy
Correspondence to Stefan Uckert, PhD, Department of Urology, Hannover Medical School, Carl-Neuberg-Str. 1, Hannover 30625, Germany. Tel: +49 511 5 32 34 37; Fax: +49 511 5 32 84 37; E-mail: E-mail: streetgang@gmx.de

KEYWORDS
Human Cavernous Arteries • Protein Kinase A • PDE5 Inhibitors • Vasculogenic Erectile Dysfunction

ABSTRACT

Introduction. The cyclic adenosine monophosphate-dependent protein kinase (cAK) is considered a key protein in the control of smooth muscle tone in the cardiovascular system. There is evidence that erectile dysfunction might be linked to systemic vascular disorders and arterial insufficiency, subsequently resulting in structural changes in the penile tissue. The expression and significance of cyclic adenosine monophosphate-dependent protein kinase (cAK) in human cavernous arteries (HCA) have not been evaluated.

AIMS

To evaluate the expression of (cAK) isoforms in HCA and examine the role of (cAK) in the cyclic adenosine monophosphate (cAMP)- and cyclic guanosine monophosphate (cGMP)-mediated control of penile vascular smooth muscle.

METHODS

The expression and distribution of phosphodiesterase type 4 (PDE4) and cAK isoforms in sections of HCA were investigated by means of immunohistochemistry and Western blot analysis. The effects of the cAK inhibitor Rp-8-CPT-cAMPS on the relaxation of isolated preparations of HCA (diameter > 100 µm) induced by rolipram, sildenafil, tadalafil, and vardenafil were studied using the organ bath technique.

MAIN OUTCOME MEASURES

Investigate the expression of cAK in relation to α-actin and PDE4 in HCA and evaluate the effects of an inhibition of cAK on the relaxation induced by inhibitors of PDE4 and PDE5 of isolated penile arteries.

RESULTS

Immunosignals specific for cAKIα, IIα, and IIβ were observed within the wall of HCA. Double stainings revealed colocalization of cAK with α-actin and PDE4. The expression of cAK isoforms was confirmed by Western blot analysis. The reversion of tension induced by inhibitors of PDE4 and PDE5 of isolated penile vascular tissue were attenuated significantly by Rp-8-CPT-cAMPS.

CONCLUSIONS

Our results demonstrate the expression of cAK isoforms in the smooth musculature of HCA and its colocalization with PDE4. A significant role for cAK in the regulation mediated by cAMP and cGMP of vascular smooth muscle tone in HCA can also be assumed. Waldkirch ES, Ückert S, Sigl K, Satzger I, Geismar U, Langnäse K, Richter K, Sohn M, Kuczyk MA, and Hedlund P. Expression of cyclic AMP-dependent protein kinase isoforms in human cavernous arteries: Functional significance and relation to phosphodiesterase type 4. J Sex Med **;**:**–**.

ACKNOWLEDGEMENT

Journal of Sexual Medicine
DIGITAL OBJECT IDENTIFIER (DOI)
10.1111/j.1743-6109.2010.01808.x About DOI

AU: Eginhard S. Waldkirch
AU: Stefan Ückert
AU: Katja Sigl
AU: Imke Satzger
AU: Ulrike Geismar
AU: Kristina Langnäse
AU: Karin Richter
AU: Michael Sohn
AU: Markus A. Kuczyk
AU: Petter Hedlund
TI: Expression of Cyclic AMP-dependent Protein Kinase Isoforms in Human Cavernous Arteries: Functional Significance and Relation to Phosphodiesterase Type 4
SO: Journal of Sexual Medicine
VL: 9999
NO: 9999
YR: 2010
CP: © 2010 International Society for Sexual Medicine
ON: 1743-6109
PN: 1743-6095
AD: Hannover Medical SchoolDivision of Surgery, Department of Urology and Urological Oncology, Hannover, Germany; ; MorphoSys AG, Martinsried, Germany; ; Hannover Medical SchoolDepartment of Dermatology and Allergology, Hannover, Germany; ; Private Dermatological Practice, Hannover, Germany; ; Otto-von-Guericke-University, Faculty of MedicineInstitute for Biochemistry and Cell Biology, Magdeburg, Germany; ; Frankfurter Diakonie-Kliniken, St. Markus Academic HospitalDepartment of Urology, Frankfurt am Main, Germany; ; University Vita Salute, Faculty of Medicine, San Raffaele HospitalDepartment of Urology, Urological Research Institute, Milan, Italy
DOI: 10.1111/j.1743-6109.2010.01808.x
US: http://dx.doi.org/10.1111/j.1743-6109.2010.01808.x